奈米鑽石的分子作用及生醫應用(Molecular Reaction and Biomedical Applications of Nanodiamonds)

在奈米鑽石(nanodiamond, ND)的分子作用及生醫應用已有多年的研究經驗,包括發現奈米鑽石具有高度的生物相容性 (Liu et al., 2007, Nanotechnology),進一步發現奈米鑽石不會干擾細胞週期及細胞分化的進行(Liu et al., 2009, Biomaterials),開發作為幹細胞的標定及追蹤之應用(Liu et al., 2009, Biomaterials; Hsu et al., 2014, Scientific Reports)。奈米鑽石具有特殊螢光特性及作用,可作為生物探針,成果被選為生物物理期刊的封面(Chao et al., 2007, Biophys J),奈米鑽石在細胞內的螢光強度可被檢測與量化(Liu et al., 2007, Nanotechnology)。奈米鑽石可結合特定生長蛋白,辨識細胞之接受器(Cheng et al., 2007, APL; Liu et al., 2008, Nanotechnology)。建立首例奈米鑽石共價連接抗癌藥物紫杉醇,作為藥物運送與癌症治療之應用(Liu et al., 2010, Nanotechnology)。在動物體內可追蹤螢光奈米鑽石(fluorescent nanodiamond, FND),開發螢光奈米鑽石作為動物體內之對比顯影劑(Vaijayanthimala et al., 2012, Biomaterials)。開發同時具有標定螢光及磁性奈米鑽石(fluorescent and magnetic nanodiamond, FMND)的癌細胞,建立首例攜帶螢光及磁性奈米鑽石標定之細胞株(FMND-bearing cancer cell lines),應用作為癌細胞或幹細胞之標定及追蹤(Lien et al., 2012, Biomaterials)

 

References

Hsieh, YH, Liu, KK, Sulake, RS, Chao, JI*, and Chen, C* (2015) Microwave-assisted efficient conjugation of nanodiamond and paclitaxel. Bioorganic & Medicinal Chemistry Letters, 25:2074-2077.

Huang, YA, Kao, CW, Liu, KK, Huang, HS, Chiang, MH, Soo, CR, Chang, HC, Chiu, TZ*, Chao, JI*, and Hwang, E* (2014) The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis. Scientific Reports, 4: 6919. 

Hsu, TC, Liu, KK, Chang, HC, Hwang, E, and Chao, JI* (2014) Labeling of neuronal differentiation and neuron cells using fluorescent nanodiamonds. Scientific Reports, 4: 5004. 

Vaijayanthimala, V§, Cheng, PY§, Yeh, SH, Liu, KK, Hsiao, CH*, Chao, JI*, and Chang, HC* (2012) The long-term stability and biocompatibility of fluorescent nanodiamond as an in vivo imaging contrast agent. Biomaterials, 33: 7794-7802. 

Lien, ZY, Hsu, TC, Liu, KK, Liao, WS, Hwang, KC, and Chao, JI* (2012) Cancer cell labeling and tracking using fluorescent and magnetic nanodiamond. Biomaterials, 33:6172-6185.

Liu, KK, Zheng WW, Wang CC, Chiu, YC, Cheng, CL, Lo YS, Chen, C, and Chao, JI* (2010) Covalent linkage of nanodiamond-paclitaxel for drug delivery and cancer therapy. Nanotechnology, 21: 315106. 

Liu, KK, Wang, CC, Cheng, CL and Chao, JI* (2009) Endocytic carboxylated nanodiamond for the labeling and tracking of cell division and differentiation in cancer and stem cells. Biomaterials, 30: 4249-4259. 

Chang, CC*, Chen, PH, Chu, HL, Lee, TC, Chou, CC, Chao, JI, Su, JY, Chen, JS, Tsai, JS, Ho, YP, Sun, KW, and Cheng, CL (2008) Laser induced popcorn-like conformational transition of nanodiamond as a nano-knife. Applied Physics Letters, 93: 3395.

Liu, KK, Chen, MF, Chen, BI, Lee, TJF, Cheng, CL, Chang, CC, Ho, YP, and Chao, JI* (2008) Alpha-bungarotoxin binding to target cell in a developing visual system by carboxylated nanodiamond. Nanotechnology, 19: 205102. 

Chao, JI, Perevedentseva, E, Chung, PH, Liu, KK, Cheng, CY, Chang, CC, and Cheng, CL* (2007) Nanometer-sized diamond particle as a probe for biolabeling. Biophysical Journal, 93: 2199-2208. 

Cheng, CY, Perevedentseva, E, Tu, JS, Chung, PH, Cheng, CL*, Liu, KK, Chao, JI*, Chen, PH, Chang, CC* (2007) Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling. Applied Physics Letters 90: 163903. 

Liu, KK, Cheng, CL, Chang, CC and Chao, JI* (2007) Biocompatible and detectable carboxylated nanodiamond on human cell. Nanotechnology, 18: 325102.